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KMID : 0381120100320040375
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Genes and Genomics
2010 Volume.32 No. 4 p.375 ~ p.384
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Analysis of genetic diversity and relationships among waxy maize inbred lines in Korea using SSR markers
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Sa Kyu-Jin
Park Jong-Yeol
Park Ki-Jin
Lee Ju-Kyong
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Abstract
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Information regarding the genetic diversity and genetic relationships among elite inbred lines is necessary to improve new cultivars in maize breeding programs. In this study, genetic diversity and genetic relationships were investigated among 84 waxy maize inbred lines using 50 SSR markers. A total of 269 alleles were identified at all the loci with an average of 5.38 and a range between 2 and 13 alleles per locus. The gene diversity values varied from 0.383 to 0.923 with an average of 0.641. The cluster tree generated using the described SSR markers recognized two major groups at 32% genetic similarity. Group I included 33 inbred lines while group II included 51 inbred lines. The clustering patterns of most of the waxy maize inbred lines did not clearly agree with their source, pedigree or geographic location. The average GS among all inbred lines was 35.7 ± 10.8. Analysis of waxy maize inbred lines collected from Korea and China at 50 SSR loci revealed higher values of average number of alleles (4.9) and gene diversity (0.638) in Korean inbred lines as compared to Chinese inbred lines (3.5 and 0.563, respectively). The information obtained from the present studies would be very useful for maize breeding programs in Korea.
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KEYWORD
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Waxy maize, SSR marker, Genetic diversity, Genetic relationship, Inbred lines, Maize breeding program
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